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1、人肌紅蛋白基因外顯子一的生物信息學(xué)分析 一. Introduction : Myoglob in, the article is composed of a peptide cha ins and a binding prote in of heme prosthetic group, is within the muscle oxygen storage protein, its oxygen saturation of the elliptic curve.Myoglobin is the main protein of skeletal muscle and cardiac muscl

2、e, whe n muscle damage, leakage to the circulating in the blood in muscle tissue, increase the serum concen tratio n of myoglob in, the in dex is used to determ ine whether muscle damage. 二. Purpose : 對 人肌紅蛋白基因外顯子一基因序列使用 “ Bioed和DNAMAN、anthe5.0及相關(guān)網(wǎng)站進(jìn)行GC含量統(tǒng)計(jì)、電 子酶切、引物設(shè)計(jì)、基因在人類基因組中的定位、電子克隆及蛋 白在細(xì)胞內(nèi)的定位、

3、氨基酸組成統(tǒng)計(jì)、分子量、等電點(diǎn)、親疏水 性、信號肽分析 ＜跨膜區(qū)域）、二級結(jié)構(gòu) ＜含保守結(jié)構(gòu)域）及三維 結(jié)構(gòu)分析，miR分析，啟動(dòng)子分析等。其中抗原表位分析、多序 列比對和進(jìn)化分析等。 三. The n ature and fun cti on of myoglob in : Nature: myoglob in = a polypeptide cha in + a prosthetic group polypeptide cha in, composed of 153 amino acid residues Prosthetic group: heme prosthetic group

4、 Molecular weight: 16, 700 Fun cti on: tran sport of oxyge n and oxyge n storage fun cti on in muscle tertiary structure of myoglob in 四. Key word:肌紅蛋白

5、行序列的基本性質(zhì)分析，限制性酶譜分析及其基因 在染色體上的定位分析。具體操作方法將根據(jù)上述各個(gè)軟件上 “ Help文件進(jìn)行 ，基因在染色體上的定位分析根據(jù)網(wǎng)頁上的操作說明進(jìn)行。 從 pubmed 蛋白質(zhì)數(shù)據(jù)庫中搜索到人的 Myoglobin和整個(gè)前體Myoglobin蛋白質(zhì)序列，分別進(jìn)行蛋白序列的基本性質(zhì)分 析 < 氨基酸組成、分子量、等電點(diǎn))，親疏水性分析，蛋白酶切位點(diǎn)分析，信號 肽序列分析，二級結(jié)構(gòu)和三維結(jié)構(gòu)分析，同源序列和進(jìn)化分析。二級結(jié)構(gòu)分析 包括結(jié)構(gòu)功能域、Motif、保守位點(diǎn)等，具體操作方法將根據(jù)上述各個(gè)軟件上 “H elp文件和網(wǎng)頁上的操作說明進(jìn)行。 Resluts:

6、1. 從NCBI中可以得到一下有關(guān) Myoglobi蛋白分子的信息： Huma n myoglob in gene, exon 1 GenBank: M10090.1 LOCUS HUMMGI1 2552 bp DNA linear PRI 07-JAN-1995 DEFINITION Huma n myoglobi n gene, exon 1. ACCESSION M10090 VERSION M10090.1 GI:187580 KEYWORDS Fok family repetitive sequenee 。direct repeat 。 myoglob in 。re

7、peat regi on 。tan dem repeat. SEGMENT 1 of 3 SOURCE Homo sapie ns (huma n> ORGANISM Homo sapie ns Eukaryota 。Metazoa 。Chordata 。 Craniata 。Vertebrata Euteleostomi 。 Mammalia 。 Eutheria 。 Euarchontoglires 。 Primates 。 Haplorrhi ni 。 Catarrhini 。 Hominidae 。 Homo. REFERENCE 1 (bases 1 to 255

8、2> AUTHORS Akaboshi,E. TITLE Cloning of the huma n myoglob in gene JOURNAL Ge ne 33 (3>, 241-249 (1985> PUBMED 2989088 COMMENT Origi nal source text: Huma n: fetal liver DNA (library of Law n et al.>, clone lambda-HMB151 。delta-thalassemia leukocyte DNA (library of Kimura et al.>, clone lam

9、bda-HMB159. Draft entry and computer-readable copy of seque nee [1] ki ndly provided by E.Akaboshi, 22-OCT-1985. A tan dem repeat seque nee is found at positi on 142-794. Positi on of the mRNA cap site was deduced from that of the seal myoglob in gene (Bla nchetot et al. 1983>. FEATURES Loc

10、atio n/Qualifiers source 1..2552 /orga ni sm="Homo sapie ns" /mol_type="genomic DNA" /db_xref="taxo n: 9606" /map="22q11.2-qter" repeat_region 1100..1750 /no te="Fok family repetitive eleme nt" prim_tra nscript 1895..>2552 /no te="myoglob in mRNA and in tr on" ge ne joi n(1965..2552,M14602

11、.1:1..2671,M14603.1:1..501> /gen e="MB" CDS joi n(1965..2059,M14602.1:1415..1637,M14603.1:355..501> /gen e="MB" /no te="myoglobi n" /codo n_ start=1 /protein_id="AAA59595.1" /db_xref="GI:386872" /tra nslati on="MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKF DKFKHLKSEDEMKASEDLKKHGATVLTALGGILK

12、KKGHHEAEIKPLAQSHATKHKIPV KYLEFISECIIQVLQSKHPGDFGADAEGAMNKALELFRKDMASNYKELGFQG" exon <1965..2059 /gen e="MB" /n ote="myoglobi n 。G00-119-378" /n umber=1 intron 2060..>2552 /gen e="MB" /no te="myoglob in intron A" ORIGIN 2 bp upstream of RsaI site. 1 gtactgtatt ttcattcctc ttagttatct ccctaaaa

13、ag actctgagtt ccttgaacac 61 aggaaggtgt tttatttgat tttgttatcc tcagcatgta gcagtgtctg acacacagta 121 ggtgctctat cactgtgaga gggatggatg gatgggtgga gttacagatg gatagaagga 181 tagatggagg gatgggtgga tgatggatgg atagatggat ggagggggga tgatgaatgg 241 agggataatg agtggatgaa tgagggaatg ggtggatgga tggatggagg g

14、atggaggaa 301 cagatagata gatggaggga tgggtgggtg atggatggat agatggatgg agggagggat 361 gatgaatgga gggataatga atggatgaat gaggggatgg gtggatggat gaatggaggg 421 atgatgggtg gatgaatgaa ttgagggatg gatggatgaa cacatggatg gatggataga 481 tggatagatg gaggaactgg tggattttgg atggatgggt ggatggatag atgaatgaat 541

15、gcctggatag acaaagagat gatggataga tgaatagatg aattaaggga tgtcggatag 601 atggagggat tgatagatgt tggatggatg ggtggtggat ggatagatga gtgaatgcat 661 ggatagacaa agagatgatg gatggatgaa ttaagggatg acagatggat ggatggatga 721 gtaactggat ggacaagtgg ataaatggat agatggttga atacctgaat ggattgaagg 781 aggatgcatg ga

16、tgtaagat aaggctaatc atcctccact ctctttcttt gcaaaaccat 841 ccacccattt actcaataaa catttattca gttcaaactt ggcacaaagc accatgtgag 901 gcccaagaga tacgtgggtt aataaaacag agctcctgcc ctcctgaaaa ctgcaaagaa 961 aggggcgtgg cttcctgagt tcaaatccca actctgccag cgactagctg tacatcagtg 1021 atgtttccct actttctctc aatta

17、aatag ggataatgtc agtacctatc acattgggag 1081 gtcttgcggg gattaaatga gttaccaaat gccaagtgtt tgggacaggg cctggcaccc 1141 agcaaagtct cttgtgagtg ctggctgcta ttatcctaat ggagaagatg gcatgaaaac 1201 caggaaatag gatgcccttt gggaagcaat gcaacaggaa cttacacaaa gaaaggaaag 1261 gaggaagcaa ttagtggtgt ctcaaaggag tatgtc

18、aaga aaaacttttc agagggaaac 1321 ctttgagcag ggtcatgaaa acaggagttc tctaagagat tgtggacttg cctgggacca 1381 cctggctata agcacaaaac catccggttc ctttctgtca cttctggcgg gtgaggggtc 1441 tctggcaaag gggcagaagg tgcgtgagag gttgcgaatg gccaggactg tcctggggcc 1501 agccggggca cctggtggcc aagcttagaa acatgacagg tcctctt

19、ggg agggctgacc 1561 gcagggagcg ttgggtttca ggctgctggc gtcggcttct gtggtgccct ttctgtcggc 1621 tatgagagtc cagacagtgc ccaacctcct ccccttcttt ccacacgcac aaccacccca 1681 ccccctgtgg cctgagctgt cctgcctcgc cacaatggca cctgccctaa aatagcttcc 1741 catgtgaggg ctagagaaag gaaaagatta gaccctccct ggatgagaga gagaaagt

20、ga 1801 aggagggcag gggaggggga cagcgagcca ttgagcgatc tttgtcaagc atcccagaag 1861 gtataaaaac gcccttggga ccaggcagcc tcaaacccca gctgttgggg ccaggacacc 1921 cagtgagccc atacttgctc tttttgtctt cttcagactg cgccatgggg ctcagcgacg 1981 gggaatggca gttggtgctg aacgtctggg ggaaggtgga ggctgacatc ccaggccatg 2041 ggc

21、aggaagt cctcatcagg taaaaggaag agattccatt gcccctgcca cccacaccct 2101 aagatcaagg gtgttcagct gcaaggtgga aagtttgcac gtggggtagg tcagttggct 2161 gcattagtta agggtgttag aacggtcact tgctttttct ttgcttttaa gtgtcaggga 2221 ttggactcag gagagggaaa ggagccattt caggctgatg tcagcagctg gaggaagcat 2281 gagaatcaaa ccta

22、ggatgc tcagagtcca ccaggaagaa ttttagaatt atagacagtc 2341 agagttaaca agggtcctga gagattttgt acagccacct ctcttacagg atgaggacaa 2401 aaagcgactg agaaggggag gacatttcca gagtcacagc tcattaaatg ctcttaaagt 2461 gtcaaggtta agacatgctc ttcaagggga gacagatctg gttctagact tggctctgcc 2521 actgagccac tgggtgacct t

23、tgggaaggt ac Official SymbolMBprovided by HGNC Official Full Namemyoglob in provided by HGNC Primary source :HGNC:6915 Locus tag :RP4-569D19.7 Seerelated : Ensembl:ENSG00000198125 。 HPRD:01170 。 MIM:160000 。 Vega:OT THUMG00000150606 Gene type:prote in cod ing Refsep status: REVIEWED Organi

24、sm : Homo sapiens Lin eage : Eukaryota。 Metazoa。 Chordata。 Crani ata。 Vertebrata。 Euteleostomi。 Mammalia。 Eutheria。 Euarchontoglires。 Primates。 Haplorrhini。 Catarrhini。 Hominidae。 Homo Also known as : PVALB Genomic context Locati on: 22q13.1 Seque nee: Chromosome: 22。NC_000022.10 (36002811..3

25、6019401, compleme nt> See MB in Epige no mics, MapViewer Chromosome 22 - NC 000022.10 4F>Q16 Genomic regions, transcripts, and products Geno micSeque nee NC_000022 chromosome 22 reference GRCh37.p13 Primary Assembly Go to nucleotide Graphics FASTA GenBank 7 / 18 8 / 18 9 / 18 10 / 18 11 / 18 12 / 18 13 / 18 14 / 18 15 / 18 16 / 18 2. 利用Primer premier5.0 軟件對myoglobin核酸序列進(jìn)行引物設(shè) 計(jì)其結(jié)果如下： Sense primer: 5' TACTCGTGCCTTTCGTACTAGGCTC 3' An ti-se nse primer: 5' ATGAGCACGGAAAGCATGATCCGAG 3' 3. 蛋白質(zhì)序列分析 利用ExPASy軟件包對該基因進(jìn)行氨基酸組成統(tǒng)計(jì)、分子 量、等電點(diǎn)等分析及磷酸化分析 19 / 18